Publications (yearly ordered)
Tiwari, A., Nikolic, N., Anagnostidis, V. and Gielen, F., (2023) Label-free analysis of bacterial growth and lysis at the single-cell level using droplet microfluidics and object detection-oriented deep learning. bioRxiv, pp.2023-06. Full text
Nikolic, N., Anagnostidis, V., Tiwari, A., Chait, R. and Gielen, F., (2023) Droplet-based methodology for investigating bacterial population dynamics in response to phage exposure. Frontiers in Microbiology, 14, p.1260196. Full text
Neun, S., Van Vliet, L., Hollfelder, F. and Gielen, F., (2022) High-throughput steady-state enzyme kinetics measured in a parallel droplet generation and absorbance detection platform. Analytical Chemistry, 94(48), pp.16701-16710. Full text
Bentley, S.A., Laeverenz-Schlogelhofer, H., Anagnostidis, V., Cammann, J., Mazza, M.G., Gielen, F. and Wan, K.Y., (2022) Phenotyping single-cell motility in microfluidic confinement. eLife, 11, p.e76519. Full text
Elvira, K.S., Gielen, F., Tsai, S.S. and Nightingale, A.M., (2022) Materials and methods for droplet microfluidic device fabrication. Lab on a Chip, 22(5), pp.859-875. Full text
Rogers, S., Zhang, C., Anagnostidis, V., Fishel, M., Gielen, F. and Scholpp, S., (2022) Cancer-associated fibroblast-derived ROR2 induces WNT/PCP activation and polarized migration in receiving gastric cancer cells. bioRxiv, pp.2022-04. Full text
Collins, S., Van Vliet, L., Gielen, F., Janeček, M., Valladolid, S.W., Poudel, C., Fusco, G., De Simone, A., Michel, C., Kaminski, C.F. and Spring, D.R., (2022) A unified in vitro to in vivo fluorescence lifetime screening platform yields amyloid β aggregation inhibitors. bioRxiv, pp.2022-03. Full text
Lindenburg L.H., Pantelejevs T., Gielen F., Zuazua-Villar P., Butz M., Rees E., Kaminski C.F., Downs J.A., Hyvönen M., Hollfelder, F. (2021). Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats. Proceedings of the National Academy of Sciences, 118(46). Full text
Howell L., Anagnostidis V., Gielen F. (2021) Multi-Object Detector YOLOv4-Tiny Enables High-Throughput Combinatorial and Spatially-Resolved Sorting of Cells in Microdroplets. Advanced Materials Technologies. Full text.
Nikiforov P.O., Hejja B., Chahwan R., Soeller C., Gielen F., Chimerel, C. (2021) Functional Phenotype Flow Cytometry: On Chip Sorting of Individual Cells According to Responses to Stimuli. Advanced Biology, p.2100220. Full text
Anagnostidis V., Sherlock B., Metz J., Mair P., Hollfelder F., Gielen F. (2020) Deep learning guided image-based droplet sorting for on-demand selection. and analysis of single cells and 3D cell cultures. Lab Chip. 20, 889.. Author URL. Full text.
Buryska T., Vasina M., Gielen F., Vanacek P., van Vliet L., Jezek J., Pilat Z., Zemanek P., Damborsky J., Hollfelder F., et al (2019) Controlled Oil/Water Partitioning of Hydrophobic Substrates Extending the Bioanalytical Applications of Droplet-Based Microfluidics. Anal Chem, 91(15), 10008-10015. Abstract. Author URL. Full text.
Kleine-Brüggeney H., van Vliet L.D., Mulas C., Gielen F., Agley C.C., Silva J.C.R., Smith A., Chalut K., Hollfelder F. (2019) Long-Term Perfusion Culture of Monoclonal Embryonic Stem Cells in 3D Hydrogel Beads for Continuous Optical Analysis of Differentiation. Small, 15(5). Abstract. Full text.
Gielen F., Colin P.Y., Mair P., Hollfelder F. (2018) Ultrahigh-Throughput Screening of Single-Cell Lysates for Directed Evolution and Functional Metagenomics. Methods Mol Biol, 1685, 297-309. Abstract. Author URL. Full text.
Mair P., Gielen F., Hollfelder F. (2017) Exploring sequence space in search of functional enzymes using microfluidic droplets. Curr Opin Chem Biol, 37, 137-144. Abstract. Author URL. Full text.
Gielen F., Butz M., Rees E.J., Erdelyi M., Moschetti T., Hyvonen M., Edel J.B., Kaminski C.F., Hollfelder F. (2017) Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets. ANALYTICAL CHEMISTRY, 89(2), 1092-1101. Author URL.
Gielen F., Hours R., Emond S., Fischlechner M., Schell U., Hollfelder F. (2016) Ultrahigh-throughput-directed enzyme evolution by absorbance-activated droplet sorting (AADS). Proc Natl Acad Sci U S A, 113(47), E7383-E7389. Abstract. Author URL. Full text.
Chen W., Avezov E., Schlachter S.C., Gielen F., Laine R.F., Harding H.P., Hollfelder F., Ron D., Kaminski C.F. (2015) A method to quantify FRET stoichiometry with phasor plot analysis and acceptor lifetime ingrowth. Biophys J, 108(5), 999-1002. Abstract. Author URL.
Gielen F., Buryska T., Van Vliet L., Butz M., Damborsky J., Prokop Z., Hollfelder F. (2015) Interfacing microwells with nanoliter compartments: a sampler generating high-resolution concentration gradients for quantitative biochemical analyses in droplets. Anal Chem, 87(1), 624-632. Abstract. Author URL.
Colin P.Y., Kintses B., Gielen F., Miton C.M., Fischer G., Mohamed M.F., Hyvönen M., Morgavi D.P., Janssen D.B., Hollfelder F., et al (2015) Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics. Nat Commun, 6 Abstract. Author URL.
Gielen F., van Vliet L., Koprowski B.T., Devenish S.R.A., Fischlechner M., Edel J.B., Niu X., deMello A.J., Hollfelder F. (2013) A fully unsupervised compartment-on-demand platform for precise nanoliter assays of time-dependent steady-state enzyme kinetics and inhibition. Anal Chem, 85(9), 4761-4769. Abstract. Author URL.
Hassan S.U., Gielen F., Niu X., Edel J.B. (2012) Controlled one dimensional oscillation of the Belousov-Zhabotinsky reaction confined within microchannels. RSC Advances, 2(16), 6408-6410. Abstract.
Gielen F., deMello A.J., Edel J.B. (2012) Dielectric cell response in highly conductive buffers. Anal Chem, 84(4), 1849-1853. Abstract. Author URL.
Niu X., Gielen F., Edel J.B., deMello A.J. (2011) A microdroplet dilutor for high-throughput screening. Nat Chem, 3(6), 437-442. Abstract. Author URL.
Gielen F., Pereira F., Demello A.J., Edel J.B. (2010) High-resolution local imaging of temperature in dielectrophoretic platforms. Anal Chem, 82(17), 7509-7514. Abstract. Author URL.
Niu X., Gielen F., deMello A.J., Edel J.B. (2009) Electro-coalescence of digitally controlled droplets. Anal Chem, 81(17), 7321-7325. Abstract. Author URL.
Gielen F., deMello A.J., Cass T., Edel J.B. (2009) Increasing the trapping efficiency of particles in microfluidic planar platforms by means of negative dielectrophoresis. J Phys Chem B, 113(5), 1493-1500. Abstract. Author URL.
Conferences
Anuj Tiwari, Vasileios Anagnostidis, Robyn Manly, Nela Nikolic, Ben Temperton and Fabrice Gielen (2023) A Label -free analysis of growth and lysis of microbial communities at the single-cell level using object detection oriented deep learning and droplet microfluidics. MicroTas2023
Anagnostidis V., Al-Saadi D. and Gielen F. (2020) AI-Assisted Microfluidic Stiffness gradient for analysis of 3D cell cultures in hydrogel Beads. Microtas2020
Bentley S., Anagnostidis V., Gielen F., Wan K.Y. (2020) Sensorimotor processing and navigation in confined microswimmers. Bulletin of the American Physical Society. Abstract.
Anagnostidis V., Sherlock B., Metz J., Mair P., Hollfelder F., and Gielen F. (2019) Deep Learning guided image-based droplet sorting for biological screenings. Microtas2019.
Collins S., Gielen F., van Vliet L., Kaminski G., Hollfelder F., Spring D. (2017) Identification and development of small molecule inhibitors of the aggregation of amyloid beta. Author URL.
Van Vliet L.D., Gielen F., Sinha A., Koprowski B.T., Edel J.B., Niu X., De Mello A.J., Hollfelder F. (2014) Droplet-on-demand platform for biochemical screening and drug discovery. Abstract.
Niu X., Gielen F., DeMello A.J., Edel J.B. (2009) A hybrid microfluidic chip for digital electro-coalescence of droplets. Abstract.